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作者:Z Shen2010被引用次数:206 — Multiplex PCR, defined as the simultaneous amplification of multiple regions of a DNA template or multiple DNA templates using more than one ...

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Multiplex PCR assays involve designing of large number of primers, hence it is required that the designed primer should be of appropriate length.
2020年5月5日 — How to Design PCR Primers for Multiplex Assays · PCR primers are generally designed to be 18 – 30 bp in length. · The melting temperature (Tm) of ...
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作者:A Kechin2020被引用次数:1 — Multiplex polymerase chain reaction (PCR) has multiple applications in molecular biology, including developing new targeted next-generation ...
Visual OMP (multiplex PCR primer assay design software) can design multiple primers and probes at the same time using a dynamic design algorithm that ...
作者:W Yan2015被引用次数:4 — Multiplex PCR primer design for simultaneous detection of multiple pathogens. Methods Mol Biol. 2015;1275:91-101. doi: 10.1007/978-1-4939-2365-6_6.
作者:R Conzemius2019被引用次数:4 — The development of multiplex polymerase chain reaction and ... Primer-BLAST) and multiplex (e.g. oli2go) primer design tools include ...
2021年5月19日 — openPrimeR was developed to provide a rational approach for evaluating and designing primers for multiplex PCR such that multiple template ...
作者:C Kreer2020被引用次数:11 — Here, we present openPrimeR, an R-based tool for evaluating and designing multiplex PCR primers. openPrimeR provides a functional and intuitive interface ...

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